Fig. 6

PyDMT1 hypomorph parasites were less sensitive to artemisinin. a Merozoite numbers of Pydmt1-C2 treated with 100 nM artemisinin (N = 80 parasites from three independent experiments, one-way ANOVA and Tukey’s multiple comparison test. ****P < 0.0001). b Merozoite numbers of Pydmt1-A1 treated with 50 nM artesunate (N = 80 parasites from three independent experiments, one-way ANOVA and Tukey’s multiple comparison test. ****P < 0.0001). c Parasitemia of BALB/c mice treated with 7.5 mg/kg artemisinin, 8 mg/kg artesunate, or 6 mg/kg chloroquine after infected with PyDMT1-HA and Pydmt1-A1 parasites. Drugs were administered when parasitemia was up to 10%. This assay is limited to just 2-day assays due to obvious immune reaction to P. yoelii 17X 5 days after infection. d Inhibition rate of BALB/c mice treated with 7.5 mg/kg artemisinin, 8 mg/kg artesunate, or 6 mg/kg chloroquine after infection with PyDMT1-HA and Pydmt1-A1 parasites (N = 5, t-test, ***P < 0.001, **P < 0.05)