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Fig. 2 | BMC Biology

Fig. 2

From: FGF-independent MEK1/2 signalling in the developing foetal testis is essential for male germline differentiation in mice

Fig. 2

FGF and MEK1/2 signalling is required for normal testis cord formation. Immunofluorescent images of XY E12.5 gonad-mesonephros tissue cultured with DMSO, 500 nM FGFRi or 500 nM MEKi for 72 h showing Sertoli (A) and germ (A,B) cell localisation. DAPI (blue), MVH (green), SMA (red: A) or Laminin (red: B) and SOX9 (cyan: A). Scale bars: top panel 100 μm, bottom panel 50 μm. Replicates: n = 3–4. A White arrows identify gaps in the Sertoli cell layer at the testis cord basement membrane; white asterisks identify Sertoli cells dispersed within the inner area of the testis cords. B White arrows identify germ cells localised outside testis cords

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BMC Biology

ISSN: 1741-7007

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