Table 2 Antibodies for flow cytometry (F) and immunofluorescence (IF)
Protein | Source and catalogue # | Species | Dilution |
|---|---|---|---|
MVH | R&D Systems, AF2030 | Goat | F: 1/100 IF: 1/400 |
DPPA4 | R&D Systems, AF3730 | Goat | F: 1/100 IF: 1/400 |
OCT4 | Santa Cruz, sc8628 | Goat | IF: 1/400 |
AMH | Santa Cruz, sc68886 | Goat | IF: 1/200 |
MVH | Cell Signalling Technology, 8761S | Rabbit | IF: 1/400 |
SOX9 | Sigma-Aldrich, AB5535 | Rabbit | F: 1/200 IF: 1/1000 |
DNMT3L | Cell Signalling Technology, 13451S | Rabbit | F: 1/100 IF: 1/100 |
STRA8 | Abcam, ab49405 | Rabbit | F: 1/500 IF: 1/400 |
Phospho-γH2AX | Cell Signalling Technology, 9718S | Rabbit | F: 1/100 IF: 1/800 |
IgG | Cell Signalling Technology, 3900S | Rabbit | Equal to primary antibody used |
Laminin | R&D Systems, L9393 | Rabbit | IF: 1/200 |
PIWIL2 | Cell Signalling Technology, 5940S | Rabbit | IF: 1/200 |
Phospho-ERK1/2 | Cell Signalling Technology, 4370S | Rabbit | IF: 1/200 |
FOXL2 | A gift from A/Prof Dagmar Wilhelm | Rabbit | IF: 1/500 |
SYCP3 | Abcam, ab97672 | Mouse | IF: 1/200 |
SMA | Sigma-Aldrich, A2547 | Mouse | IF: 1/1000 |
NR2F2 | R&D Systems, PP-H7147-00 | Mouse | IF: 1/400 |
SOX2 | Cell Signalling Technologies, 4900S | Mouse | IF: 1/100 |