Fig. 2
From: Identification of FtfL as a novel target of berberine in intestinal bacteria
BBR directly binds to PaFtfL with high affinity. A The scheme of target identification of BBR in P. anaerobius using probe-based target identification strategy. B BBR-biotinylated probe (BBP) specifically binds to a 60-kDa protein. BBP (100 μM) was incubated with cell lysate of P. anaerobius with or without BBR (1 mM); the SDS-PAGE analysis of probe-bound proteins showed a band of about 60 kDa specific to BBR (red box). The identities of the band were determined by LC–MS. C The pull-down assay show BBR binds with PaFtfL, using purified PaFtfL (3 μM) and BBP; BBP (100 μM) was incubated with PaFtfL with or without BBR (1 mM). D BBR directly binds PaFtfL. The kinetics 1:1 binding model was applied for fitting the data to determine the binding affinity. E Effect of BBR on PaFtfL enzymatic activity. The dose-dependent inhibitory curve was fit to calculate the IC50. Data shown are mean ± S.D. from three independent experiments. The one-way ANOVA test was used for statistical analysis, p < 0.01