Fig. 4

Polλ enhances editing outcomes of 1bp deletions and suppresses the generation of >1bp deletions in HEC-1-B cells. There is a significant decrease of frequencies of 1bp deletions but an increase of frequencies of 2–20bp and 21–100bp deletions upon PolL knockdown at the MAZ (A–C), MeCP2 (D–F), PARP1 (G–I), PRDM5 (J–L), and YY1 (M–O) loci. As positive controls, the trends of small deletions upon Ku70/80-knockdown are similar as those of Polλ-knockdown (see Additional file 5: Table S3, n = 2 replicates, mean ± SEM)