Fig. 4

Strain promotes vascular growth even with VEGFR-2 inhibition. a HUVECs were grown in fibrin gels with magnetic beads and treated with either no treatment (NT) control media, 25 ng/mL VEGF (V), vehicle control (Veh), and/or 3 µM SU5416 (SU). Samples were either cultured without external magnetic stimulation (No Magnet) or over a rotating magnetic field (Magnet). Gels were stained for VE-cadherin, and representative images are shown. Scale bar = 500 µm. b Vessel growth was quantified using AngioTool, and total vessel length was normalized to explant or imaged area. * p < 0.05 versus No Magnet of same treatment group. Data shown as average + SEM, n = 3 samples. Samples were compared with ANOVA, followed by post hoc Tukey HSD tests