Fig. 5

The gating polarity of K525N is switched by E4031 and cisapride. a Representative step currents of K525N with or without 3 μM E4031. Green arrows indicate peak currents, and red arrowheads indicate steady-state currents. b I-V curves of peak currents (n≥6) and c steady-state currents (n≥6) of K525N with or without E4031. d Representative step currents of K525N with or without 10 μM cisapride. Green arrows indicate peak currents, and red arrowheads indicate steady-state currents. e I-V curves of peak currents (n≥6) and f steady-state currents (n≥6) of K525N with or without cisapride. G-V curves of K525N with or without g E4031 and h cisapride. Error bars represent mean ± SEM