Fig. 1
From: Dysregulation of innate immune signaling in animal models of spinal muscular atrophy
The proteomes and transcriptomes of Drosophila Smn hypomorphs provide overlapping evidence for innate immune activation. A A rectangular cartoon and an AlphaFold model of the relative positions of conserved domains of the Drosophila SMN protein and the location of the patient-derived missense mutations used here. B Principal component analysis of total protein abundances in the Smn transgenic lines. Smn lines: WT (SmnX7/X7,Flag-SmnTg:WT); T205I (SmnX7/X7,Flag-SmnTg:T205I), Tyrosine (T) to Isoleucine (I); and V72G (SmnX7/X7,Flag-SmnTg:T205I), Valine (V) to Glycine (G). C Venn diagram of overlapping protein differences in T205I and V72G relative to WT. D Volcano plot of protein differences in the T205I line relative to WT. Proteins associated with innate immunity are indicated by larger dots. E Volcano plot of protein differences in the V72G line relative to WT, and proteins associated with innate immunity are labeled as in D. Dashed vertical bars in D and E indicate a Log2 FC ratio of ± 0.58, and the horizontal dashed line corresponds to q-value = 0.05. F Comparison of T205I proteome (y-axis) with T205I transcriptome (x-axis). The proteome and transcriptome are relative to the WT genotype. G Comparison of V72G proteome (y-axis) with V72G transcriptome (x-axis). As in F, the proteome and transcriptome are relative to WT. H V72G proteome (y-axis) versus SmnX7/D null transcriptome (x-axis). The differential gene expression of the SmnX7/D transcriptome is relative to Oregon-R. Note that the total (Ribo-minus) RNA-seq data [18] on the Smn hypomorphs were originally generated with the intent to measure non-coding RNA levels (specifically, spliceosomal snRNAs) and are therefore not as deep as one might like to use for measuring mRNAs, particularly the lowly-expressed ones. The Smn null datasets were polyA-selected and are thus better able to detect changes in mRNA levels