Fig. 3From: Dysregulation of innate immune signaling in animal models of spinal muscular atrophySmn missense mutants exhibit elevated melanotic masses. A–C Melanotic mass (MM) data for wandering third instar larvae expressing Smn missense mutations. The data in each panel are a different measure of the melanotic mass phenotypes of the same set of larvae. A Percent of larvae with one or more melanotic mass. Individual data points are the percent of larvae with MMs, 10 larvae per data point. B The average number of melanotic masses per animal. Data points show the number of MMs in each animal. Number (N) = 50 larvae for each genotype. C Qualitative size scoring of the largest melanotic mass in each larva. D Representative images of MMs in animals expressing Smn missense mutations. Bars show the mean, and error bars show the standard error of the mean. Asterisks indicate p-values relative to WT: * < 0.05; ** < 0.01; and *** < 0.001. E Graph showing correlation between the overall phenotypic severity of SMA-causing Smn missense mutations and the number of MMs per animal (from panel B). SMA-like phenotypic severity scores were assigned for each allele (zero being the mildest) based on previously published viability and locomotor assays [20]. Dotted line shows linear regression between data points along with a goodness-of-fit coefficient (R 2)Back to article page